Effects of salvianolic acid and notoginseng triterpenes on angiogenesis in EA-hy926 cells in vitro / 中国天然药物

AIM@#To investigate the different effects of salvianolic acid and notoginseng triterpenes on proliferation, angiogenesis and expression of vascular endothelial growth factor in EA-hy926 cells in vitro.@*METHODS@#EA-hy926 cells were cultured in vitro. Salvianolic acid and notoginseng triterpenes at concentrations of 0.4, 0.8 and 1.2 mg·L(-1) were used to culture EA-hy926 cells. EA-hy926 cells in a blank control group were grown in culture solution only. Viability of cells was assessed by CCK-8, and after treated for 12 h, capillary-like structures were examined. After 24 h culture, the expression of VEGF was detected by real-time PCR.@*RESULTS@#Salvianolic acid at 0.4, 0.8 mg·L(-1), the same as notoginseng triterpenes, increased VEGF content in EA-hy926 cells. Expression of VEGF protein in the salvianolic acid at 1.2 mg·L(-1) group, was up-regulated as compared with notoginseng triterpenes group (P < 0.05).@*CONCLUSION@#Salvianolic acid and notoginseng triterpenes can promote EA-hy926 cell proliferation, angiogenesis and expression of VEGF protein. This analysis also provided evidence that salvianolic acid had the better effects as compared with notoginseng triterpenes.

An epidemiological investigation on a food-born outbreak of noroviru caused by Sydney 2012 G II.4 strain / 中华流行病学杂志

<p><b>OBJECTIVE</b>To identify the source of infection, route of transmission and risk factors related to a cluster of acute gastroenteritis cases in a university of Guangzhou.</p><p><b>METHODS</b>Cases were identified according to the definition. Descriptive epidemiological approaches and case-control study designs were employed in the analysis. All the samples were tested for norovirus by RT-PCR. Positive samples were subjected to both nucleotide sequence and homology analysis.</p><p><b>RESULTS</b>A total of 141 cases related to norovirus gastroenteritis were identified in January 8 to 21, 2013, with the attack rate as 8.5 per thousand (141/16,600). The peak in morbidity was seen on January 8 to 9. No clustering was found in different classes or dormitories. Results from the case-control study revealed that early cases were infected in Restaurant A (OR = 3.46, 95% CI: 1.07-11.16) and the cold shredded chicken set meal (OR = 17.82, 95% CI: 4.46-78.17) served at lunch (OR = 4.34, 95% CI: 1.18 -17.37) on January 7 was under suspicion. A total of 266 samples, including rectal swabs from the patients and kitchen wokers, leftover food and environmental swabs, were collected. Twenty-one samples (collected from 17 persons) were positive for norovirus by RT-PCR. About 29.6% (8/27) of the kitchen workers in the Restaurant A were tested positive for the virus. The pathogen was identified as the new norovirus genotype II.4 variant, termed Sydney 2012. The virus strains isolated from the patients among student and staff and the kitchen workers were 100% identical in their nucleotide sequence.</p><p><b>CONCLUSION</b>This was the first reported acute gastroenteritis outbreak caused by the new norovirus genotype II.4 variant, Sydney 2012, which showed that the food was contaminated by the asymptomatic kitchen workers who carried the virus.</p>

Effect of clinical pathways based on integrative medicine for patients with chronic heart failure: a multi-center research / 中国中西医结合杂志

<p><b>OBJECTIVE</b>To assess a multi-center study effectiveness of clinical pathways based on integrative medicine (IM) for chronic heart failure (CHF) patients.</p><p><b>METHODS</b>A combined method of historical control study and clinical study on concurrent control was used. After the standard management for clinical pathways was carried out in four hospitals at home, the effects on hospitalization days, medical expenses, clinical efficacy, patient satisfaction, and quality of life were assessed.</p><p><b>RESULTS</b>Results of non-concurrent historical control study showed that: the hospital stay was significantly shorter in the pathways group than in the retrospective group (12.59 days vs 18.44 days), and the total cost of hospitalization was significantly reduced in the pathways group (yen 9 051.90 vs yen 11 978.40), showing statistical difference (P < 0.01). Moreover, the effect on the heart function was better in the pathways group than in the retrospective group (the markedly effective rate: 45.60% vs 21.90%; the total effective rate: 96.80% vs 86.10%), showing statistical difference (P < 0.01). Results of clinical study on concurrent control showed that the hospital stay was significantly shorter in the pathways group than in the control group (11.19 days vs 13.21 days), showing statistical difference (P < 0.05). The average total cost of hospitalization was significantly lower in the pathways group than in the control group (yen 8 656.80 vs yen 11 609.70), showing statistical difference (P < 0.01). As for clinical efficacy of Chinese medical syndrome, the total effective rate was higher in the pathways group than in the control group (97.10% vs 93.62%), showing statistical difference (P < 0.05). The markedly effective rate of heart function was better in the pathways group than in the control group, showing statistical difference (49.30% vs 38.30%, P < 0.05). The overall satisfaction was higher in the pathways group than in the conventional group (P < 0.01). There was no statistical difference in the mortality within 3 months after discharge from hospital, and the readmission rate due to heart failure between the two groups (P > 0.05). But there was statistical difference in the quality of life (P < 0.05).</p><p><b>CONCLUSION</b>The pathway could shorten the hospitalization time, decrease the cost of hospitalization, improve the clinical efficacy, improve patients' quality of life and satisfaction, therefore, it could be spread nationwide.</p>

A case-control study of an influenza A (H1N1) outbreak in a hospital / 中华预防医学杂志

<p><b>OBJECTIVE</b>This study aimed to explore the epidemiological factors of an influenza A (H1N1) outbreak in a hospital.</p><p><b>METHODS</b>General data were collected via face-to-face interview and telephone survey. Total 132 individuals including medical and nursing staffs (37), in-patients (39) and patients' family members (56) who were exposed to the pediatric surgery ward during August 11 - 18, 2009, were investigated. The case group included 35 cases according to the diagnostic criteria for influenza A (H1N1). The other 97 persons were grouped as control. A case-control study was then conducted to explore the epidemic factors, and layering analysis was applied to determine the interactions among these factors.</p><p><b>RESULTS</b>The overall incidence in this study was 26.5% (35/132), which included 12 confirmed and 23 suspected cases, and there was no severe case. The first case was a child with the influenza-like symptoms before admission on August 11. The onsets of these cases were during August 7 - 17. The cases were distributed in 9 of 13 rooms, and there was no room aggregation in the cases distribution (χ(2) = 0.00, P > 0.05). Twelve of 25 oropharyngeal swabs were influenza A (H1N1) nucleic acid positive. The case-control study showed that exposure to the enema room accounted for 93.10% (27/29) in cases and 72.73% (48/66) in control; OR = 5.06, 95%CI = 1.01 - 34.23), long time exposure to ward was 71.43% (25/35) in cases and 44.33% (43/97) in control; OR = 3.14, 95%CI = 1.27 - 7.90), and short distance contact with the nurse LIU (76.46% (26/34) in cases and 50.52% (49/97) in control; OR = 3.18, 95%CI = 1.22 - 8.54) were the risk factors. However, keeping the window open (27.59% (8/29) in cases and 68.18% (45/66) in control; OR = 0.14, 95%CI = 0.05 - 0.39) and hand washing (25.71% (9/35) in cases and 76.29% (74/97) in control; OR = 0.11, 95%CI = 0.04 - 0.28) were the protective factors. The longer time exposure to ward had the higher risk (ratios of cases to control were 4:20 (0 - 1 day), 6:34 (2 - 4 days) and 25:43 (≥ 5 days); χ(2)(trend) = 5.737, P < 0.05). In contrast, hand washing with more frequencies (ratios of cases to control were 26:23 (0 - 1 time one day), 7:9 (2 - 3 times one day) and 2:65 (≥ 4 times one day); χ(2)(trend) = 37.136, P < 0.01) and the longer time window opening (ratios of cases to control were 21:21 (no), 4:13 (a few) and 4:32 (often); χ(2)(trend) = 13.830, P < 0.01) had the lower risk. Nevertheless, layering analysis excluded long time exposure to ward from the risk factors (for individuals with more frequent hand washing, 6.90% (2/29) exposed in cases, 7.14% (1/14) exposed in control, OR = 0.97, 95%CI = 0.06 - 29.51; for individuals keeping window open, 21.21% (7/33) exposed in cases, 8.33% (1/12) exposed in control, OR = 2.55, 95%CI = 0.26 - 60.87), indicating the main risk factors in this outbreak were exposure to the enema room and short distance contagion with the infected nurse.</p><p><b>CONCLUSION</b>The influenza A (H1N1) outbreak in this hospital was induced by an inpatient infected with influenza A (H1N1) virus before admission. Infected medical staffs keeping on work and exposure to the same place, e.g.the enema room in this study might spread the influenza A (H1N1) virus, and frequent hand washing and keeping the window open are the most effective and economic methods to prevent influenza A (H1N1) infection.</p>

Regulatory effects of 3 kinds of antifibrotic cytokines on activity of transforming growth factor-?_1 gene promoter / 第二军医大学学报

T polymorphism,was selected as putative promoter.The recombinant constructions containing-1328-+812 of TGF-?_1 gene and CAT reporter gene(phTGF2.14T,phTGF2.14C)were constructed and transfected into HepG2 cells with liposomal trans- fection method,then the transfected HepG2 cells were treated with IL-10(4 ng/ml),HGF(10 ng/ml)or IFN-?(20 ng/ml). Reporter gene activity was analyzed by ELISA.Results:Reporter gene activity in cells transfected with phTGF2.14C was sig- nificantly higher than those transfected with phTGF2.14T(P